ngf  (Cedarlane)

Journal: Developmental cell

Article Title: Prenylation of Axonally Translated Rac1 Controls NGF-Dependent Axon Growth

doi: 10.1016/j.devcel.2020.05.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Sheep anti-NGF , Cedarlane , Cat# CLMCNET-031.

Techniques: Virus, Recombinant, Sequencing, RNAscope, Multiplex Assay, Isolation, TA Cloning, Membrane, Bicinchoninic Acid Protein Assay, shRNA, Cloning, Plasmid Preparation, Software

Journal: Nature Communications

Article Title: Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency

doi: 10.1038/s41467-020-20031-4

Figure Lengend Snippet: a RT-qPCR analysis for transcription across VLT and ORF63 loci in latently VZV-infected human iPSC-derived sensory neurons (HSN) cultures ( n = 3). Data on individual HSN culture experiments are shown as unique symbols. b , c Latently VZV-infected HSN cultures were depleted of neurotrophic factors (NGF [nerve growth factor], GDNF [glial-derived neurotrophic factor], BDNF [brain derived neurotrophic factor] and NT-3 [neurotrophin-3]) and treated with anti-NGF antibody (Ab) for 14 days. In total, n = 40 independent cultures were subjected to reactivation stimuli. b Representative examples of a HSN cultures showing complete reactivation (2/40) and early/abortive reactivation (38/40) by infectious focus forming assay after transferring HSN onto ARPE-19 cells. c Representative examples of viral gene expression in cultures showing complete reactivation ( n = 1 shown; white circle) and early/abortive reactivation ( n = 5 shown; colored triangles). d , e HSN cultures were treated with d anisomycin ( n = 6), or e DMSO as solvent control ( n = 3) at both the somal and axonal compartment for 1 h, washed twice and cultured for 7 days before RT-qPCR analysis. Data on individual HSN culture experiments are shown as unique symbols and/or colors. Only VLT exon 1–2 is shown as representative of VLT. Source data are provided as a Source Data file ( a – e ).

Article Snippet: Anti-alpha tubulin mAb (clone B-5-1–2; Sigma-Aldrich) and sheep anti-NGF pAb (EMD Millipore) are commercially available.

Techniques: Quantitative RT-PCR, Infection, Derivative Assay, Focus Forming Assay, Transferring, Expressing, Cell Culture

Journal: Cell reports

Article Title: Coupled Control of Distal Axon Integrity and Somal Responses to Axonal Damage by the Palmitoyl Acyltransferase ZDHHC17

doi: 10.1016/j.celrep.2020.108365

Figure Lengend Snippet: (A) Cultured DRG neurons infected with the indicated lentiviruses were subjected to TD for 2.5 h or left unstimulated and then lysed and blotted as indicated. Lower panel shows ZDHHC17 (arrow) and anti-NGF antibody used for TD, detected by secondary antibody (asterisk). (B) Quantified data from (A); n = 5; ***p < 0.001; two-way ANOVA; Bonferroni post hoc test (virus p = 0.0003; TD p < 0.0001; interaction p = 0.0003). (C) Zdhhc17 CKO blocks DLK-dependent signaling. Top: experimental setup is shown. Bottom: immunostained retinal flat mounts from Zdhhc17 f/f mice injected with the indicated AAVs and fixed 33 h post-ONC are shown. Right panels: overlay of columns 1 to 2 are shown. (D) Quantified GFP + /p-c-Jun + cells from (C). ***p < 0.001; two-way ANOVA; Bonferroni post hoc test; n = 4 (virus p < 0.0001; ONC p < 0.0001; interaction p< 0.0001). (E) Zdhhc17 CKO blocks ONC-induced caspase-3 (Casp3) activation. Top: experimental setup is shown. Bottom: immunostained retinal flat mounts from Zdhhc17 f/f mice injected with the indicated AAVs and fixed 6 days post-ONC are shown. Right panels: overlay of columns 1 to 2 is shown. Filled arrowheads, GFP/Casp3 double-positive RGC; empty arrowhead, GFP-positive/Casp3-negative RGC. Insets, magnified images of cells highlighted by arrowheads. (F) GFP + /Casp3 + cells from (E); n = 5–6. ***p < 0.001; two-way ANOVA; Bonferroni post hoc test. n = 5–6 (virus p = 0.0013; ONC p < 0.0001; interaction p = 0.0009).

Article Snippet: Sheep anti-NGF , CedarLane , Catalog # CLMCNET-031, RRID: AB_10060173.

Techniques: Cell Culture, Infection, Injection, Activation Assay

Journal: Cell reports

Article Title: Coupled Control of Distal Axon Integrity and Somal Responses to Axonal Damage by the Palmitoyl Acyltransferase ZDHHC17

doi: 10.1016/j.celrep.2020.108365

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Sheep anti-NGF , CedarLane , Catalog # CLMCNET-031, RRID: AB_10060173.

Techniques: Plasmid Preparation, Modification, shRNA, Cell Culture, Recombinant, Purification, Mutagenesis, Software